custom peptide synthesis service |
Posted: February 16, 2017 |
In the quantitative proteomics research, several MS-based methodologies for relative quantification have been introduced for comparison of different proteomes from collected biological samples. Meanwhile, MS-based methods for absolute quantification of specific proteins have been developed to accurately determine the protein concentrations. According to the guidelines for bioanalytical methods, the establishment and validation of accurate analytic proposals require standard compounds of high purity for calibrating and quality controls. Currently the dominant quantitative strategy is usually a combination of shotgun method and isotope dilution strategy. The targeted proteins in the complicated biological samples would release free peptide fragments induced by specific enzymatic cleavage, and the stable peptides with unique primary sequences in the digest mixture would be utilized as surrogates for corresponding parent proteins, so the small-molecular peptides can be quantified to estimate the protein concentration. Synthetic methods used in Creative Proteomics: Fmoc and Boc methodologies are both employed, using solid and solution phase reactions. Boc-chemistry solid phase peptide synthesis allows us to synthesise difficult sequences, and gives a greater flexibility in the synthesis of modified peptides. Fmoc chemistry is most suitable for simple peptides and sequences prone to oxidation. Quality assurance files supplied after synthetic: All peptides are supplied with COA, RP-HPLC and Mass Spec data. CHN analysis (available, will be online soon), amino acid analysis and N-terminal sequencing can be supplied at an extra cost. View example data: COA, RP-HPLC and Mass Spec. What purity should I order? • The most common MS grade purity requested is >95%; More: http://www.creative-proteomics.com/services/customized-synthesized-peptide-proteins.htm
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